Determination of Enzyme Activity

GLP and GMP certified enzyme activity assays for natural and engineered enzymes, enzymatic biomarkers, and enzyme inhibitors.

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Kymos Group provides enzymatic activity determination assays under GLP/GMP conditions in both bioanalytical and CMC projects for natural and engineered enzymes, enzymatic biomarkers, and enzyme inhibitors in biological and formulation samples.

With our extensive experience, we can provide services for:

Therapeutic enzymes
Enzyme-inhibitors
Enzyme and protein research in need of drug-level quantification and enzyme kinetics.

Our methodology

We determine enzyme activity levels by providing a synthetic substrate to the enzyme and measuring the product and turnover rate by means of a microtiter reader for absorption, luminescence, and fluorescence detection in different applications:

Kinetic measurements of enzyme activity
Enzyme/substrate specificity analysis
Inhibitor efficacy and inhibition reversibility screenings

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Bioanalysis by Enzymatic Activity

GLP-certified Enzymatic activity determination in biological samples for Preclinical and Clinical studies. This enzymatic activity format is adapted from EMA’s Immunological guidelines for this purpose, given the lack of specific guidelines for the determination of enzyme activity.

Enzyme-based drugs or drugs involved in enzymatic reactions are quantified by means of their biocatalytic activity in two different ways:

Endpoint measurements: The enzyme reaction is stopped after a defined time period.
Real-time enzyme kinetics: The enzyme reaction is monitored per time unit.

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