{"id":27236,"date":"2019-04-28T13:38:09","date_gmt":"2019-04-28T11:38:09","guid":{"rendered":"https:\/\/kymos.com\/news\/electrophorese-dans-le-controle-de-la-qualite-des-produits-biologiques\/"},"modified":"2025-12-03T18:58:50","modified_gmt":"2025-12-03T17:58:50","slug":"electrophorese-dans-le-controle-de-la-qualite-des-produits-biologiques","status":"publish","type":"post","link":"https:\/\/kymos.com\/fr\/news\/electrophorese-dans-le-controle-de-la-qualite-des-produits-biologiques\/","title":{"rendered":"\u00c9lectrophor\u00e8se dans le contr\u00f4le de la qualit\u00e9 des produits biologiques"},"content":{"rendered":"

L’\u00e9lectrophor\u00e8se est une technique analytique largement utilis\u00e9e pour la d\u00e9termination de la puret\u00e9 et l’identification des prot\u00e9ines th\u00e9rapeutiques. Cette technique repose sur la migration des prot\u00e9ines sous l’effet d’un champ \u00e9lectrique, en fonction de leur mobilit\u00e9 \u00e9lectrophor\u00e9tique. Selon le type d’\u00e9lectrophor\u00e8se, diff\u00e9rents attributs de qualit\u00e9 de la prot\u00e9ine peuvent \u00eatre mesur\u00e9s. La sensibilit\u00e9, la robustesse et le d\u00e9bit d\u00e9pendent du format utilis\u00e9 ; les formats classiques \u00e0 gel plat\/vertical sont progressivement remplac\u00e9s par les formats capillaires plus r\u00e9cents, en raison de leurs avantages intrins\u00e8ques. Les analyses \u00e9lectrophor\u00e9tiques, sous diff\u00e9rents types et formats, sont actuellement incluses comme m\u00e9thode de contr\u00f4le dans les certificats d’analyse pour les tests par lots de prot\u00e9ines th\u00e9rapeutiques. <\/span><\/p>\n

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De nombreuses prot\u00e9ines telles que les cytokines, les facteurs sanguins, les hormones, les anticorps monoclonaux (mAbs) et les prot\u00e9ines de fusion sont test\u00e9es \u00e0 l’aide de diff\u00e9rentes techniques d’\u00e9lectrophor\u00e8se comme outil pour identifier et tester leur int\u00e9grit\u00e9.<\/span><\/p>\n

Selon les principes appliqu\u00e9s \u00e0 la technique, on peut distinguer plusieurs types d’\u00e9lectrophor\u00e8se :<\/span><\/p>\n

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  • SDS-PAGE ( \u00e9lectrophor\u00e8se<\/b> sur<\/b> gel de polyacrylamide<\/b> en pr\u00e9sence de<\/b> dod\u00e9cylsulfate<\/b> de<\/b> sodium<\/b> ) : elle est r\u00e9alis\u00e9e sous forme de gel plat ou vertical et permet de s\u00e9parer les prot\u00e9ines en fonction de leur poids mol\u00e9culaire.<\/span><\/li>\n
  • PAGE ( \u00e9lectrophor\u00e8se<\/b> sur<\/b> gel<\/b> de polyacrylamide<\/b> ) : elle est r\u00e9alis\u00e9e sous forme de gel plat ou vertical et permet de s\u00e9parer les prot\u00e9ines en fonction de leurs charges.<\/span><\/li>\n
  • IEF ( focalisation<\/b> iso\u00e9lectrique<\/b> ) : elle est r\u00e9alis\u00e9e sur gel plat ou vertical et permet de s\u00e9parer les prot\u00e9ines en fonction<\/b> de leurs points iso\u00e9lectriques.<\/span><\/li>\n
  • L’ \u00e9lectrophor\u00e8se<\/b> capillaire<\/b> en pr\u00e9sence de<\/b> dod\u00e9cylsulfate de<\/b> sodium<\/b> (CE-SDS) est r\u00e9alis\u00e9e par \u00e9lectrophor\u00e8se capillaire et permet de s\u00e9parer les prot\u00e9ines en fonction de leur masse mol\u00e9culaire. Cette technique pr\u00e9sente une sensibilit\u00e9, un d\u00e9bit et une reproductibilit\u00e9 sup\u00e9rieurs \u00e0 ceux de l’\u00e9lectrophor\u00e8se SDS-PAGE classique. <\/span><\/li>\n
  • L’ \u00e9lectrophor\u00e8se<\/strong> capillaire<\/b> de<\/b> zone (CZE) est r\u00e9alis\u00e9e gr\u00e2ce \u00e0 la technologie d’\u00e9lectrophor\u00e8se capillaire et permet de s\u00e9parer les prot\u00e9ines en fonction de leur mobilit\u00e9 \u00e9lectrophor\u00e9tique. Cette technique pr\u00e9sente une sensibilit\u00e9, un d\u00e9bit et une reproductibilit\u00e9 sup\u00e9rieurs \u00e0 l’\u00e9lectrophor\u00e8se sur gel de polyacrylamide (PAGE) classique. De plus, elle poss\u00e8de un large \u00e9ventail d’applications, l’analyse des glycanes \u00e9tant l’une des principales applications dans la caract\u00e9risation des anticorps monoclonaux (AcM). <\/span><\/li>\n
  • cIEF ( focalisation<\/b> iso\u00e9lectrique<\/b> capillaire<\/b> ) : cette technique utilise l\u2019\u00e9lectrophor\u00e8se capillaire et permet de s\u00e9parer les prot\u00e9ines selon leur point iso\u00e9lectrique. Elle offre une meilleure sensibilit\u00e9, un d\u00e9bit plus \u00e9lev\u00e9 et une meilleure<\/b> reproductibilit\u00e9 que l\u2019IEF classique. <\/span><\/li>\n
  • icIEF ( imagerie<\/b> par<\/b> focalisation iso\u00e9lectrique<\/b> capillaire<\/b> ) : cette technique utilise l\u2019\u00e9lectrophor\u00e8se capillaire et permet de s\u00e9parer les prot\u00e9ines selon leur point iso\u00e9lectrique. Similaire \u00e0 la cIEF ,<\/b> elle offre cependant un meilleur d\u00e9bit. <\/span><\/li>\n<\/ul>\n

    Toutes ces techniques \u00e9lectrophor\u00e9tiques sont disponibles chez Kymos gr\u00e2ce \u00e0 diff\u00e9rents instruments et sont actuellement appliqu\u00e9es au contr\u00f4le qualit\u00e9 des prot\u00e9ines th\u00e9rapeutiques innovantes et biosimilaires :<\/span><\/p>\n

      \n
    • Chambres d’\u00e9lectrophor\u00e8se et alimentations \u00e9lectriques de diff\u00e9rentes tailles et de diff\u00e9rents fabricants (Bio-Rad, etc.) pour l’\u00e9lectrophor\u00e8se classique<\/span><\/li>\n
    • Syst\u00e8mes d’\u00e9lectrophor\u00e8se Multiphor II de GE Healthcare Life Sciences pour l’\u00e9lectrophor\u00e8se \u00e0 plat<\/span><\/li>\n
    • densitom\u00e8tre calibr\u00e9 GS-900 de Bio-Rad pour convertir les gels d’\u00e9lectrophor\u00e8se en images num\u00e9riques et traiter les donn\u00e9es avec le logiciel appropri\u00e9.<\/span><\/li>\n
    • Syst\u00e8mes d’\u00e9lectrophor\u00e8se capillaire Beckman\/Sciex PA800 pour CE.SDS, CZE et cIEF.<\/span><\/li>\n
    • iCE3 de Protein Simple pour icIEF.<\/span><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":6,"featured_media":27237,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[267],"tags":[],"class_list":["post-27236","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cmc"],"acf":[],"yoast_head":"\n\u00c9lectrophor\u00e8se dans le contr\u00f4le de la qualit\u00e9 des produits biologiques | Kymos<\/title>\n<meta name=\"description\" content=\"D\u00e9couvrez les techniques \u00e9lectrophor\u00e9tiques disponibles chez Kymos et comment nous pouvons vous aider dans votre contr\u00f4le qualit\u00e9.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link 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Dou","image":{"@type":"ImageObject","inLanguage":"fr-FR","@id":"https:\/\/secure.gravatar.com\/avatar\/263d7ff1c7891f5821a5075a1a362a1e513db5931dc882455e887fe1c1ccf924?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/263d7ff1c7891f5821a5075a1a362a1e513db5931dc882455e887fe1c1ccf924?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/263d7ff1c7891f5821a5075a1a362a1e513db5931dc882455e887fe1c1ccf924?s=96&d=mm&r=g","caption":"Joan Puig de Dou"},"description":"Active in the pharmaceutical marketing for over 30 years President of CataloniaBIO&HealthTech CEO and Qualified Person at Kymos Leader of Kymos team, guaranteeing a trustable, ethical and scientific correct editorial publications. Experience Joan Puig de Dou started his career at Laboratorios Menarini first as scientist later as Qualified Person and finally as Operations General Manager. 2010 he entered Kymos where he became CEO in 2015. 2022 Joan Puig de Dou was elected as president of CataloniaBio&HealthTech to represent the Catalonian biotech industry nationally and internationally. Expertise Joan Puig de Dou has extensive experience in writing scientific content adopting a didactic approach that fits any reader\u2019s level of expertise. He publishes regularely on Kymos\u2019 webpage, social media and in the specialized press. Education He graduated from Universitat de Barcelona as Pharmacist and from IESE Business School\u2019s Programa Diecci\u00f3n General (PDG) Want to know him better? Read his interview in PharmaBoardrooom in January 2019 and more about his presidency of CataloniaBio at Parc Sc\u00edentific de Barcelona.","url":"https:\/\/kymos.com\/fr\/news\/author\/joan-puig-de-dou\/"}]}},"_links":{"self":[{"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/posts\/27236","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/comments?post=27236"}],"version-history":[{"count":0,"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/posts\/27236\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/media\/27237"}],"wp:attachment":[{"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/media?parent=27236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/categories?post=27236"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/kymos.com\/fr\/wp-json\/wp\/v2\/tags?post=27236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}